cell lines hct116 american type culture collection  (ATCC)

Journal: Cell chemical biology

Article Title: Target identification and mechanism of action of picolinamide and benzamide chemotypes with antifungal properties

doi: 10.1016/j.chembiol.2017.12.007

Figure Lengend Snippet: Structures and IC 50 values for active compounds IC 50 values were determined in a cell based growth assay with the heterozygous SEC14 deletion strain against a wildtype control, with a luminescent cell viability assay on HCT116 cells and in an in vitro lipid transfer assay, as indicated. “nd” indicates “not determined”.

Article Snippet: BL21- CodonPlus (DE3)-RIL Agilent Technologies #230245 Fungal Strains S.c. BY4743 OpenBiosyste ms Cat# YSC1050 S.c. BY4743Δ8 Dominic Hoepfner’s lab BY4743Δ8 S.c heterozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1055 S.c homozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1056 S.c. CTY1-1A Bankaitis’ lab CTY1-1A S.c. CTY159 Bankaitis’ lab CTY159 S.c. CTY303 Bankaitis’ lab CTY303 C.albicans American Type Culture Collection (ATCC) Cat# 10231 C.glabrata American Type Culture Collection (ATCC) Cat# 2001 A.brasiliensis American Type Culture Collection (ATCC) Cat# 16404 C.neoformans Royal Botanic Gardens, Kew Cat# DSM70219 Mammalian Cell Lines Human colorectoral carcinoma cell line HCT116 American Type Culture Collection (ATCC) Cat# CCL-247 Recombinant DNA Plasmid: SEC14 CG Bankaitis’ lab pDK247 Plasmid: SEC14 CA Bankaitis’ lab pDK9 Plasmid: SEC14 CA M154V, C154V Bankaitis’ lab pDK265 Plasmid: pDR195- SEC14 Bankaitis’ lab pDR195 Plasmid: YCplac33- SFH1 Schaaf’s lab N/A Plasmid: YCplac33- SFH1 * Schaaf’s lab N/A Plasmid: YCplac33- SEC14 Schaaf’s lab N/A Plasmid: BYInt Hoepfner’s lab pBYInt_LEU Chemicals and Recombinant Proteins Compounds 1- 48 Waldman’s lab Compound number as listed in Table S1 His 8 -Sec14 Schaaf’s lab N/A His 8 -Sec14 Y151H Bankaitis’ lab N/A His 8 -Sec14 V154G Bankaitis’ lab N/A His 8 -Sec14 V155A Bankaitis’ lab N/A His 8 -Sec14 S173L Bankaitis’ lab N/A His 8 -Sec14 S201G Bankaitis’ lab N/A Software and Algorithms Tibco Spotfire TIBCO Software Inc. http://www.spotfire.tibco.com GOLD Suite The Cambridge Crystallographic Data Centre (CCDC) https://www.ccdc.cam.ac.uk Pymol Schrödigner https://pymol.org XDS Kabsch et al., Max-Plack Institute, Heidelberg http://xds.mpimf-heidelberg.mpg.de Phaser McCoy et al., University of Cambridge http://www.ccp4.ac.uk/html/phaser.html Coot Emsley et al., University of Cambridge https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ MOE Chemical Computing Group https://www.chemcomp.com/ Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Deposited Data S.c. Sec14::compound 2 co-crystal structure Protein databank PDB 6F0E ( http://www.rcsb.org/pdb/explore/explore.do?structureId=6F0E ) Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING

Techniques: Growth Assay, Control, Cell Viability Assay, In Vitro

Journal: Cell chemical biology

Article Title: Target identification and mechanism of action of picolinamide and benzamide chemotypes with antifungal properties

doi: 10.1016/j.chembiol.2017.12.007

Figure Lengend Snippet: Growth inhibitory concentrations against pathogenic fungi Minimal inhibitory concentrations on solid medium have been determined following the Clinical Laboratory Standards Institute reference in triplicates.

Article Snippet: BL21- CodonPlus (DE3)-RIL Agilent Technologies #230245 Fungal Strains S.c. BY4743 OpenBiosyste ms Cat# YSC1050 S.c. BY4743Δ8 Dominic Hoepfner’s lab BY4743Δ8 S.c heterozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1055 S.c homozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1056 S.c. CTY1-1A Bankaitis’ lab CTY1-1A S.c. CTY159 Bankaitis’ lab CTY159 S.c. CTY303 Bankaitis’ lab CTY303 C.albicans American Type Culture Collection (ATCC) Cat# 10231 C.glabrata American Type Culture Collection (ATCC) Cat# 2001 A.brasiliensis American Type Culture Collection (ATCC) Cat# 16404 C.neoformans Royal Botanic Gardens, Kew Cat# DSM70219 Mammalian Cell Lines Human colorectoral carcinoma cell line HCT116 American Type Culture Collection (ATCC) Cat# CCL-247 Recombinant DNA Plasmid: SEC14 CG Bankaitis’ lab pDK247 Plasmid: SEC14 CA Bankaitis’ lab pDK9 Plasmid: SEC14 CA M154V, C154V Bankaitis’ lab pDK265 Plasmid: pDR195- SEC14 Bankaitis’ lab pDR195 Plasmid: YCplac33- SFH1 Schaaf’s lab N/A Plasmid: YCplac33- SFH1 * Schaaf’s lab N/A Plasmid: YCplac33- SEC14 Schaaf’s lab N/A Plasmid: BYInt Hoepfner’s lab pBYInt_LEU Chemicals and Recombinant Proteins Compounds 1- 48 Waldman’s lab Compound number as listed in Table S1 His 8 -Sec14 Schaaf’s lab N/A His 8 -Sec14 Y151H Bankaitis’ lab N/A His 8 -Sec14 V154G Bankaitis’ lab N/A His 8 -Sec14 V155A Bankaitis’ lab N/A His 8 -Sec14 S173L Bankaitis’ lab N/A His 8 -Sec14 S201G Bankaitis’ lab N/A Software and Algorithms Tibco Spotfire TIBCO Software Inc. http://www.spotfire.tibco.com GOLD Suite The Cambridge Crystallographic Data Centre (CCDC) https://www.ccdc.cam.ac.uk Pymol Schrödigner https://pymol.org XDS Kabsch et al., Max-Plack Institute, Heidelberg http://xds.mpimf-heidelberg.mpg.de Phaser McCoy et al., University of Cambridge http://www.ccp4.ac.uk/html/phaser.html Coot Emsley et al., University of Cambridge https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ MOE Chemical Computing Group https://www.chemcomp.com/ Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Deposited Data S.c. Sec14::compound 2 co-crystal structure Protein databank PDB 6F0E ( http://www.rcsb.org/pdb/explore/explore.do?structureId=6F0E ) Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING

Techniques: Concentration Assay

Journal: Cell chemical biology

Article Title: Target identification and mechanism of action of picolinamide and benzamide chemotypes with antifungal properties

doi: 10.1016/j.chembiol.2017.12.007

Figure Lengend Snippet: CONTACT FOR REAGENT AND RESOURCE SHARING

Article Snippet: BL21- CodonPlus (DE3)-RIL Agilent Technologies #230245 Fungal Strains S.c. BY4743 OpenBiosyste ms Cat# YSC1050 S.c. BY4743Δ8 Dominic Hoepfner’s lab BY4743Δ8 S.c heterozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1055 S.c homozygous, genome-wide deletion collection OpenBiosyste ms Cat# YSC1056 S.c. CTY1-1A Bankaitis’ lab CTY1-1A S.c. CTY159 Bankaitis’ lab CTY159 S.c. CTY303 Bankaitis’ lab CTY303 C.albicans American Type Culture Collection (ATCC) Cat# 10231 C.glabrata American Type Culture Collection (ATCC) Cat# 2001 A.brasiliensis American Type Culture Collection (ATCC) Cat# 16404 C.neoformans Royal Botanic Gardens, Kew Cat# DSM70219 Mammalian Cell Lines Human colorectoral carcinoma cell line HCT116 American Type Culture Collection (ATCC) Cat# CCL-247 Recombinant DNA Plasmid: SEC14 CG Bankaitis’ lab pDK247 Plasmid: SEC14 CA Bankaitis’ lab pDK9 Plasmid: SEC14 CA M154V, C154V Bankaitis’ lab pDK265 Plasmid: pDR195- SEC14 Bankaitis’ lab pDR195 Plasmid: YCplac33- SFH1 Schaaf’s lab N/A Plasmid: YCplac33- SFH1 * Schaaf’s lab N/A Plasmid: YCplac33- SEC14 Schaaf’s lab N/A Plasmid: BYInt Hoepfner’s lab pBYInt_LEU Chemicals and Recombinant Proteins Compounds 1- 48 Waldman’s lab Compound number as listed in Table S1 His 8 -Sec14 Schaaf’s lab N/A His 8 -Sec14 Y151H Bankaitis’ lab N/A His 8 -Sec14 V154G Bankaitis’ lab N/A His 8 -Sec14 V155A Bankaitis’ lab N/A His 8 -Sec14 S173L Bankaitis’ lab N/A His 8 -Sec14 S201G Bankaitis’ lab N/A Software and Algorithms Tibco Spotfire TIBCO Software Inc. http://www.spotfire.tibco.com GOLD Suite The Cambridge Crystallographic Data Centre (CCDC) https://www.ccdc.cam.ac.uk Pymol Schrödigner https://pymol.org XDS Kabsch et al., Max-Plack Institute, Heidelberg http://xds.mpimf-heidelberg.mpg.de Phaser McCoy et al., University of Cambridge http://www.ccp4.ac.uk/html/phaser.html Coot Emsley et al., University of Cambridge https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ MOE Chemical Computing Group https://www.chemcomp.com/ Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Deposited Data S.c. Sec14::compound 2 co-crystal structure Protein databank PDB 6F0E ( http://www.rcsb.org/pdb/explore/explore.do?structureId=6F0E ) Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING

Techniques: Genome Wide, Recombinant, Plasmid Preparation, Software, Cell Viability Assay